{"id":93591,"date":"2025-02-27T11:43:05","date_gmt":"2025-02-27T10:43:05","guid":{"rendered":"https:\/\/port.lukasiewicz.gov.pl\/researches\/"},"modified":"2025-12-15T11:46:38","modified_gmt":"2025-12-15T10:46:38","slug":"researches","status":"publish","type":"page","link":"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/researches\/","title":{"rendered":"Researches"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"93591\" class=\"elementor elementor-93591 elementor-64767\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-da43f22 e-flex e-con-boxed e-con e-parent\" data-id=\"da43f22\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-5c0633a e-con-full e-flex e-con e-child\" data-id=\"5c0633a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c6ced08 elementor-widget elementor-widget-heading\" data-id=\"c6ced08\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Biology of Astrocytes Research Group<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-63a9fff elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"63a9fff\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;horizontal&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; class=\\&quot;e-font-icon-svg e-fas-caret-down\\&quot; viewBox=\\&quot;0 0 320 512\\&quot; xmlns=\\&quot;http:\\\/\\\/www.w3.org\\\/2000\\\/svg\\&quot;&gt;&lt;path d=\\&quot;M31.3 192h257.3c17.8 0 26.7 21.5 14.1 34.1L174.1 354.8c-7.8 7.8-20.5 7.8-28.3 0L17.2 226.1C4.6 213.5 13.5 192 31.3 192z\\&quot;&gt;&lt;\\\/path&gt;&lt;\\\/svg&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-horizontal e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-63a9fff\" class=\"elementor-nav-menu\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93607\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/\" class=\"elementor-item\">About Us<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93606\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/researches\/\" class=\"elementor-item\">Researches<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93604\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/projects\/\" class=\"elementor-item\">Projects<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93603\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/publications\/\" class=\"elementor-item\">Publications<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93605\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/contact\/\" class=\"elementor-item\">Contact<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-63a9fff\" class=\"elementor-nav-menu\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93607\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/\" class=\"elementor-item\" tabindex=\"-1\">About Us<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93606\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/researches\/\" class=\"elementor-item\" tabindex=\"-1\">Researches<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93604\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/projects\/\" class=\"elementor-item\" tabindex=\"-1\">Projects<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93603\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/publications\/\" class=\"elementor-item\" tabindex=\"-1\">Publications<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-93605\"><a href=\"https:\/\/port.lukasiewicz.gov.pl\/en\/rd-centers\/p4health-center\/biology-of-astrocytes-research-group\/contact\/\" class=\"elementor-item\" tabindex=\"-1\">Contact<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c5f0afb e-flex e-con-boxed e-con e-parent\" data-id=\"c5f0afb\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-17ddff5 e-con-full e-flex e-con e-child\" data-id=\"17ddff5\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7f806b5 elementor-widget elementor-widget-heading\" data-id=\"7f806b5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Research topics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-991a89e e-con-full e-flex e-con e-child\" data-id=\"991a89e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-73444f2 elementor-widget elementor-widget-text-editor\" data-id=\"73444f2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Astrocytes are known for their diverse and essential functions, such as neurotransmitter recycling, regulation of synaptic transmission, and control of brain metabolism. These tasks are modulated by systemic signals, including hormones. Our team has discovered that astrocytes are a direct transcriptional target of glucocorticoids in the brain, and we have identified metabolic pathways in astrocytes that are controlled through activation of the glucocorticoid receptor.<br>  <\/p><p>Moreover, we have demonstrated that this signaling pathway is necessary for the central effects of acute and chronic stress. Interestingly, many members of the glucocorticoid receptor\u2013dependent pathway have consistently been identified as dysregulated in transcriptomic studies of brain samples from patients with various psychiatric disorders.<br> <\/p><p>In our current research, we analyze the involvement of selected pathways and genes in the neurobiological correlates of disturbances specific to defined brain circuits associated with psychiatric phenotypes.<br><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-27cb739 e-con-full elementor-hidden-tablet elementor-hidden-mobile e-flex e-con e-child\" data-id=\"27cb739\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-cffda5b e-flex e-con-boxed e-con e-parent\" data-id=\"cffda5b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-e8ea425 e-con-full e-flex e-con e-child\" data-id=\"e8ea425\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2737858 elementor-widget elementor-widget-heading\" data-id=\"2737858\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Transgenic Mouse Models<br><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-98832ea elementor-widget elementor-widget-text-editor\" data-id=\"98832ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"53\" data-end=\"740\">In our research, we use advanced transgenic mouse models that allow precise genetic manipulations in selected cell populations and at defined developmental stages or during adulthood. We apply conditional gene knockouts using the Cre-ERT2\/loxP system, which enables temporally and spatially controlled gene inactivation.<br> <\/p><p class=\"\" data-start=\"53\" data-end=\"740\">To deliver genetic material, we also use viral vectors. Additionally, through in utero electroporation, we can introduce genetic manipulations into specific cell types not only in mice but also in other species.<br> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0957425 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"0957425\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-635808d e-flex e-con-boxed e-con e-parent\" data-id=\"635808d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-6413c31 e-con-full e-flex e-con e-child\" data-id=\"6413c31\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ad79848 elementor-widget elementor-widget-heading\" data-id=\"ad79848\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Imaging<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a1329cd elementor-widget elementor-widget-text-editor\" data-id=\"a1329cd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"742\" data-end=\"1318\">We use modern in vivo imaging techniques that allow real-time monitoring of glial and neuronal cell activity in behaving animals. We employ one-photon and two-photon microscopy to image calcium signaling in astrocytes. <\/p><p class=\"\" data-start=\"742\" data-end=\"1318\">Additionally, using fiber photometry, it is possible to record cell activity in real time while simultaneously analyzing the behavior of individual animals. We also perform long-term in vitro imaging of cells to monitor physiological and metabolic changes.<br> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-89f14d6 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"89f14d6\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a056220 e-flex e-con-boxed e-con e-parent\" data-id=\"a056220\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-781ee05 e-con-full e-flex e-con e-child\" data-id=\"781ee05\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d455ccb elementor-widget elementor-widget-heading\" data-id=\"d455ccb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Omics<br><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-516dbff elementor-widget elementor-widget-text-editor\" data-id=\"516dbff\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"1320\" data-end=\"1715\">We have optimized protocols for isolating various cell types from the mouse brain, enabling precise molecular analyses. We have also developed effective methods for isolating astrocyte nuclei from freshly frozen human samples. RNA-seq and single-nucleus RNA-seq analyses allow for deep profiling of gene expression in selected cell populations.<br>  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-f4d992d e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"f4d992d\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c2f90ce e-flex e-con-boxed e-con e-parent\" data-id=\"c2f90ce\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-8b8aae9 e-con-full e-flex e-con e-child\" data-id=\"8b8aae9\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f00126f elementor-widget elementor-widget-heading\" data-id=\"f00126f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Behavior<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1a5538c elementor-widget elementor-widget-text-editor\" data-id=\"1a5538c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"1717\" data-end=\"2281\">In our research, we use automated systems to track spontaneous behavior in multiple animals simultaneously. We work with rodent stress models, such as chronic social defeat stress and early maternal separation.<br> <\/p><p class=\"\" data-start=\"1717\" data-end=\"2281\">We analyze depressive phenotypes using a social interaction test in three chambers, sucrose preference tests, and dark\u2013light box tests. We also conduct cognitive tests, such as the Y-maze or elevated plus maze, and we analyze circadian rhythms using running-wheel cages.<br> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-072344a e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"072344a\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-111bbdb e-flex e-con-boxed e-con e-parent\" data-id=\"111bbdb\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-7edb441 e-con-full e-flex e-con e-child\" data-id=\"7edb441\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-bf01c74 elementor-widget elementor-widget-heading\" data-id=\"bf01c74\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Cell Cultures<br><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-20f371c elementor-widget elementor-widget-text-editor\" data-id=\"20f371c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"2283\" data-end=\"2620\">We use primary cultures of mouse astrocytes and neurons, as well as human astrocytes derived from induced pluripotent stem cells (iPSCs). We also perform co-cultures of neurons and astrocytes, which allow us to analyze interactions between these cell types in controlled in vitro conditions.<br> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-64fd7f0 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"64fd7f0\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c973019 e-flex e-con-boxed e-con e-parent\" data-id=\"c973019\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-7a1f801 e-con-full e-flex e-con e-child\" data-id=\"7a1f801\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-39aec7f elementor-widget elementor-widget-heading\" data-id=\"39aec7f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Metabolic Measurements<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3cd7ecf elementor-widget elementor-widget-text-editor\" data-id=\"3cd7ecf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"2622\" data-end=\"3078\">For cellular metabolism analysis, we use the Seahorse system, which enables measurement of glycolytic rate, oxygen consumption, and energy substrate utilization. We also use the TMRE indicator to assess mitochondrial membrane potential, as well as live-cell imaging to measure glycolytic rate. In addition, we perform long-term imaging of genetically encoded metabolic indicators both in vitro and in vivo.<br>  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-ba0d457 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"ba0d457\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-732fb09 e-flex e-con-boxed e-con e-parent\" data-id=\"732fb09\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-044e338 e-con-full e-flex e-con e-child\" data-id=\"044e338\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-856b806 elementor-widget elementor-widget-heading\" data-id=\"856b806\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Molecular Genetics<br><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7ed69ff elementor-widget elementor-widget-text-editor\" data-id=\"7ed69ff\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"3080\" data-end=\"3446\">In our laboratory, we routinely use classical gene-cloning methods, including Gateway, Gibson, and Golden Gate techniques. We also use bacterial artificial chromosomes and viral vectors for genetic material delivery. We conduct gene editing using the CRISPR\/Cas9 method, as well as gene knockdown using shRNA and siRNA.<br>  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b09ae51 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"b09ae51\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-52c9f73 e-flex e-con-boxed e-con e-parent\" data-id=\"52c9f73\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-a4a0629 e-con-full e-flex e-con e-child\" data-id=\"a4a0629\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c85e3c6 elementor-widget elementor-widget-heading\" data-id=\"c85e3c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Standard Analytical Methods<br><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-70fce23 elementor-widget elementor-widget-text-editor\" data-id=\"70fce23\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"\" data-start=\"3448\" data-end=\"3761\">To assess protein and RNA expression, we use immunocytochemistry, immunohistochemistry, Western blotting, Southern blotting, and Golgi staining. We also perform ELISA analyses and RNAscope, which enables precise localization and quantitative assessment of RNA transcripts in tissues. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-25e6354 e-con-full cut-off-wrapper-90 e-flex e-con e-child\" data-id=\"25e6354\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Biology of Astrocytes Research Group Research topics Astrocytes are known for their diverse and essential functions, such as neurotransmitter recycling, regulation of synaptic transmission, and control of brain metabolism. These tasks are modulated by systemic signals, including hormones. Our team has discovered that astrocytes are a direct transcriptional target of glucocorticoids in the brain, and [&hellip;]<\/p>\n","protected":false},"author":218,"featured_media":0,"parent":93593,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","inline_featured_image":false,"footnotes":""},"class_list":["post-93591","page","type-page","status-publish","hentry"],"acf":{"page_icon":null},"publishpress_future_action":{"enabled":false,"date":"2026-04-28 17:20:53","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"translation_priority","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pages\/93591","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/users\/218"}],"replies":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/comments?post=93591"}],"version-history":[{"count":1,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pages\/93591\/revisions"}],"predecessor-version":[{"id":93592,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pages\/93591\/revisions\/93592"}],"up":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pages\/93593"}],"wp:attachment":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=93591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}