{"id":89909,"date":"2025-06-24T15:53:03","date_gmt":"2025-06-24T13:53:03","guid":{"rendered":"https:\/\/port.lukasiewicz.gov.pl\/?post_type=projects&p=89909"},"modified":"2025-12-16T18:56:19","modified_gmt":"2025-12-16T17:56:19","slug":"constitutive-autoreactivity-of-gamma-delta-t-cells-as-a-beneficial-mechanism-of-epidermal-barrier-control-opus_tgd","status":"publish","type":"projects","link":"https:\/\/port.lukasiewicz.gov.pl\/en\/projekty-badawcze\/constitutive-autoreactivity-of-gamma-delta-t-cells-as-a-beneficial-mechanism-of-epidermal-barrier-control-opus_tgd\/","title":{"rendered":"Constitutive Autoreactivity of Gamma Delta T Cells as a Beneficial Mechanism of Epidermal Barrier Control – OPUS_TGD"},"content":{"rendered":"\t\t
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Project funded by the National Science Centre (NCN)<\/strong> under the \u201cOPUS 8\u201d<\/strong> call<\/p>

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Project value: PLN 1,287,623.00<\/strong>
Funding amount: PLN 1,287,623.00<\/strong>
Project duration: 30\/09\/2015 \u2013 30\/09\/2018<\/strong>
Project Manager: Grzegorz Chodaczek, PhD<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Autoreactive T lymphocytes are typically associated with debilitating autoimmune diseases<\/strong>, in which the immune system attacks the body\u2019s own tissues. However, the applicant demonstrated\u2014using cutaneous \u03b3\u03b4 T cells<\/strong>\u2014that autoreactivity can actually be beneficial<\/strong> and play a critical role<\/strong> in maintaining tissue homeostasis<\/strong> and regulating wound healing<\/strong> (Chodaczek et al., Nature Immunology<\/em>, 2012).<\/p>

Through an innovative combination of static and intravital microscopy techniques<\/strong>, it was shown that \u03b3\u03b4 T lymphocytes in the healthy mouse skin<\/strong> are constitutively activated<\/strong> due to continuous stimulation of the \u03b3\u03b4 T-cell receptor (TCR \u03b3\u03b4)<\/strong> within immunological synapses<\/strong>. Contrary to the prevailing view that TCR \u03b3\u03b4 recognizes undefined stress-induced antigens<\/strong>, these findings suggest the presence of a ligand for TCR \u03b3\u03b4<\/strong> that exists even in healthy tissue<\/strong>.<\/p>

In controlling wound healing, cutaneous \u03b3\u03b4 T cells utilize not only TCR<\/strong> but also NKG2D<\/strong>, a receptor characteristic of NK cells<\/strong>, which recognizes proteins induced by tissue injury<\/strong>. However, in vitro studies<\/strong> have not definitively established which signaling pathway\u2014TCR or NKG2D\u2014plays a greater role in the effector functions<\/strong> of \u03b3\u03b4 T cells and their ability to stimulate wound repair<\/strong>. The analysis is complicated by the fact that the natural ligand for the skin TCR \u03b3\u03b4 receptor remains unidentified<\/strong>.<\/p>

Given the unexpected discovery<\/strong> of constitutive \u03b3\u03b4 T-cell synapses<\/strong>, it is still unclear whether in vitro findings<\/strong> accurately reflect the in vivo situation<\/strong>. The proposed project was designed to address these unresolved questions and aims to elucidate the role of autoreactive TCR \u03b3\u03b4<\/strong> and the significance of cutaneous \u03b3\u03b4 T cells<\/strong> in maintaining epidermal homeostasis<\/strong>.<\/p>

The central research hypothesis<\/strong> assumes that constitutive synapses<\/strong> serve as sites of intracellular or extracellular material transfer<\/strong>, with such material\u2014stored in cytoplasmic granules<\/strong>\u2014either secreted toward keratinocytes<\/strong> or internalized by the lymphocyte. It is further hypothesized that the dynamics of intracellular transport<\/strong> differ between healthy and stressed tissue<\/strong>, depending on the activation signal<\/strong> delivered by either TCR \u03b3\u03b4<\/strong> or NKG2D<\/strong>.<\/p>

The research tasks described in this project are designed to test these hypotheses. Previous studies revealed an accumulation of GM1 ganglioside-containing vesicles<\/strong> within the synapses. Since gangliosides<\/strong> are known to induce keratinocyte differentiation<\/strong>, it is hypothesized that \u03b3\u03b4 T cells release these vesicles to promote wound healing<\/strong>. This concept is entirely novel<\/strong> in the context of \u03b3\u03b4 T-cell biology.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Project funded by the National Science Centre (NCN) under the \u201cOPUS 8\u201d call \u00a0 Project value: PLN 1,287,623.00Funding amount: PLN 1,287,623.00Project duration: 30\/09\/2015 \u2013 30\/09\/2018Project Manager: Grzegorz Chodaczek, PhD Autoreactive T lymphocytes are typically associated with debilitating autoimmune diseases, in which the immune system attacks the body\u2019s own tissues. However, the applicant demonstrated\u2014using cutaneous \u03b3\u03b4 […]<\/p>\n","protected":false},"featured_media":94100,"menu_order":0,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","inline_featured_image":false,"footnotes":""},"categories":[727,747],"grupy_badawcze":[614],"pozostale_projekty":[],"class_list":["post-89909","projects","type-projects","status-publish","format-standard","has-post-thumbnail","hentry","category-finished","category-nsc","grupy_badawcze-grupa-badawcza-immunoterapii"],"acf":{"kierownik_projektu":["Dr. hab. Grzegorz Chodaczek"],"dofinansowanie":"Narodowe Centrum Nauki","grupa_badawcza":["Immunotherapy Research Group"],"nazwa_projektu":"","tematyka_projektu":"","opis_projektu":"","dofinanowanie":"National Science Centre"},"publishpress_future_action":{"enabled":false,"date":"2026-05-01 19:09:28","action":"category","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/89909","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects"}],"about":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/projects"}],"version-history":[{"count":5,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/89909\/revisions"}],"predecessor-version":[{"id":95487,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/89909\/revisions\/95487"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media\/94100"}],"wp:attachment":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=89909"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/categories?post=89909"},{"taxonomy":"grupy_badawcze","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/grupy_badawcze?post=89909"},{"taxonomy":"pozostale_projekty","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pozostale_projekty?post=89909"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}