{"id":90026,"date":"2025-10-19T08:00:43","date_gmt":"2025-10-19T06:00:43","guid":{"rendered":"https:\/\/port.lukasiewicz.gov.pl\/?post_type=projects&p=90026"},"modified":"2025-12-16T13:33:36","modified_gmt":"2025-12-16T12:33:36","slug":"spectroscopic-and-microscopic-techniques-for-nano-probing-modeling-and-recognition-of-interactions-between-red-blood-cells-and-vascular-endothelial-cells-at-the-molecular-level-kit","status":"publish","type":"projects","link":"https:\/\/port.lukasiewicz.gov.pl\/en\/projekty-badawcze\/spectroscopic-and-microscopic-techniques-for-nano-probing-modeling-and-recognition-of-interactions-between-red-blood-cells-and-vascular-endothelial-cells-at-the-molecular-level-kit\/","title":{"rendered":"Spectroscopic and Microscopic Techniques for Nano-Probing, Modeling, and Recognition of Interactions Between Red Blood Cells and Vascular Endothelial Cells at the Molecular Level – KIT"},"content":{"rendered":"\t\t
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Project funded by the National Science Centre (NCN)<\/strong> under the \u201cSONATA BIS-10\u201d<\/strong> competition<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Project number: UMO-2020\/38\/E\/ST4\/00197<\/strong>
Project value: 485,040.00 PLN<\/strong>
Funding amount: 485,040.00 PLN<\/strong>
Project duration: 25\/04\/2022 \u2013 24\/04\/2027<\/strong>
Project manager: Katarzyna Marzec, PhD<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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The project aims to design and apply spectroscopic and microscopic methodologies<\/strong> for nano-scale probing, modeling, and recognition<\/strong> of interactions between red blood cells (RBCs)<\/strong> and vascular endothelial cells (ECs)<\/strong> at the molecular level<\/strong>.<\/p>

A multi-modal analytical approach<\/strong> will be employed, combining:<\/p>

  • Fourier Transform Infrared Spectroscopy (FTIR)<\/strong> and nano-IR spectroscopy<\/strong>,<\/p><\/li>

  • Raman Spectroscopy (RS)<\/strong>, including Surface-Enhanced Raman Spectroscopy (SERS)<\/strong> and Resonance Raman Spectroscopy (RRS)<\/strong>,<\/p><\/li>

  • Atomic Force Microscopy (AFM)<\/strong> and Scanning Near-Field Optical Microscopy (SNOM)<\/strong>,
    alongside complementary reference techniques<\/strong>.<\/p><\/li><\/ul>

    The project comprises five main research tasks<\/strong>:<\/p>

    1. Nano-IR membrane probing<\/strong> \u2013 Development of a nano-IR-based methodology for analyzing biochemical alterations in intact membranes of RBCs and ECs, as well as in membranes of RBCs interacting with EC membranes.<\/p><\/li>

    2. SERS membrane interaction modeling<\/strong> \u2013 Creation of SERS-based analytical tools to study membrane changes and model interactions between metal surfaces, RBCs, and ECs.<\/p><\/li>

    3. AFM and RS characterization of microvesicles (MVs)<\/strong> \u2013 Nano-scale microscopic analysis of cell membranes and microvesicles from RBCs and ECs, including those produced during cell\u2013cell interactions, with additional spectroscopic assessment of MV biochemistry.<\/p><\/li>

    4. RRS cross-talk tracking<\/strong> \u2013 Spectroscopic examination of RBC\u2013EC communication mediated by nitric oxide (NO)<\/strong> molecules in both in vitro<\/strong> and ex vivo<\/strong> systems.<\/p><\/li>

    5. Multimodal interaction description<\/strong> \u2013 Integration of data obtained from nano-IR, SERS, AFM, RS, and RRS<\/strong> to correlate biochemical and structural profiles of RBCs and ECs and assess how RBC alterations influence EC function.<\/p><\/li><\/ol>

      The project investigates single intact cells<\/strong> and interacting RBC\u2013EC systems<\/strong> using both in vitro human cell models<\/strong> (human RBCs and human aortic endothelial cells \u2013 HAECs) and ex vivo approaches<\/strong> (cells isolated from murine models of atherosclerosis, diabetes,<\/strong> and heart failure<\/strong>).<\/p>

      In the initial phase, nano-IR spectroscopy will be applied to study membrane alterations<\/strong> in intact cells and isolated membranes under disease-related<\/strong> and age-related conditions<\/strong>. Subsequent experiments will focus on label-free SERS<\/strong> and tag-based SERS<\/strong> for the specific detection of adhesive proteins<\/strong> involved in RBC\u2013EC interactions, employing metal nanoparticle tags<\/strong>.<\/p>

      Further analyses will focus on microvesicle-mediated intercellular communication<\/strong>, where MVs will be examined using AFM, RS, and RRS<\/strong>, supported by flow cytometry, morphological analysis, and ektacytometry<\/strong>. The final stage will explore RBC\u2013EC cross-talk mechanisms<\/strong>, with emphasis on hemoglobin (Hb) adduct changes<\/strong> inside RBCs in contact with ECs, using RRS<\/strong> supported by EPR, UV-Vis spectroscopy, and blood gas analysis<\/strong>.<\/p>

      Ultimately, the multimodal interaction model<\/strong> developed in this project will provide a comprehensive molecular-level description<\/strong> of RBC\u2013EC interactions<\/strong>, helping to predict vascular endothelial status<\/strong> and disease-related alterations<\/strong> based on spectroscopic and microscopic cellular profiles.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

      Project funded by the National Science Centre (NCN) under the \u201cSONATA BIS-10\u201d competition Project number: UMO-2020\/38\/E\/ST4\/00197Project value: 485,040.00 PLNFunding amount: 485,040.00 PLNProject duration: 25\/04\/2022 \u2013 24\/04\/2027Project manager: Katarzyna Marzec, PhD VIEW PROJECT The project aims to design and apply spectroscopic and microscopic methodologies for nano-scale probing, modeling, and recognition of interactions between red blood cells […]<\/p>\n","protected":false},"featured_media":94100,"menu_order":0,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","inline_featured_image":false,"footnotes":""},"categories":[747],"grupy_badawcze":[614],"pozostale_projekty":[],"class_list":["post-90026","projects","type-projects","status-publish","format-standard","has-post-thumbnail","hentry","category-nsc","grupy_badawcze-grupa-badawcza-immunoterapii"],"acf":{"kierownik_projektu":["Dr. hab. Grzegorz Chodaczek"],"dofinansowanie":"Narodowe Centrum Nauki","grupa_badawcza":["Immunotherapy Research Group"],"nazwa_projektu":"","tematyka_projektu":"","opis_projektu":"","dofinanowanie":"National Science Centre"},"publishpress_future_action":{"enabled":true,"date":"2027-04-24 00:00:00","action":"category","newStatus":"draft","terms":[727],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/90026","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects"}],"about":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/projects"}],"version-history":[{"count":5,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/90026\/revisions"}],"predecessor-version":[{"id":95457,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/90026\/revisions\/95457"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media\/94100"}],"wp:attachment":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=90026"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/categories?post=90026"},{"taxonomy":"grupy_badawcze","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/grupy_badawcze?post=90026"},{"taxonomy":"pozostale_projekty","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pozostale_projekty?post=90026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}