{"id":92989,"date":"2025-06-24T11:05:24","date_gmt":"2025-06-24T09:05:24","guid":{"rendered":"https:\/\/port.lukasiewicz.gov.pl\/?post_type=projects&p=92989"},"modified":"2025-12-19T11:34:33","modified_gmt":"2025-12-19T10:34:33","slug":"multiparametric-characterization-of-epithelial-homeostasis-control-in-the-reproductive-tract-by-gamma-delta-t-cells-using-high-resolution-intravital-microscopy-sonata_tgd","status":"publish","type":"projects","link":"https:\/\/port.lukasiewicz.gov.pl\/en\/projekty-badawcze\/multiparametric-characterization-of-epithelial-homeostasis-control-in-the-reproductive-tract-by-gamma-delta-t-cells-using-high-resolution-intravital-microscopy-sonata_tgd\/","title":{"rendered":"Multiparametric Characterization of Epithelial Homeostasis Control in the Reproductive Tract by Gamma-Delta T Cells Using High-Resolution Intravital Microscopy \u2013 SONATA_TGD"},"content":{"rendered":"\t\t
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Project funded by the National Science Centre (NCN) under the \u201cSONATA BIS 4<\/strong>\u201d call<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Total project value: PLN 1,997,904.00<\/strong>
Total funding: PLN 1,340,184.00<\/strong>
Project implementation period: 01\/07\/2015 \u2013 30\/06\/2020<\/strong>
Principal Investigator: Dr. Grzegorz Chodaczek<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Project consortium:<\/h3>

  • Wroc\u0142aw Research Centre EIT+ Ltd. \u2013 Consortium Leader<\/p><\/li>

  • Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences<\/p><\/li><\/ul>

    The aim of the project is to analyze the mechanisms of immune control in the reproductive mucosa, with particular emphasis on the activity of lymphocytes expressing the \u03b3\u03b4 T-cell receptor (\u03b3\u03b4 TCR). The study employs innovative imaging techniques based on high-resolution confocal microscopy and in vivo intravital microscopy. To achieve a comprehensive characterization of immune surveillance\u2014essential for maintaining epithelial homeostasis during infection or injury\u2014the proposed in situ cross-sectional analysis will be complemented with additional advanced ex vivo techniques.<\/p>

    Among the many cell types involved in mucosal surveillance, \u03b3\u03b4 T cells play a particularly important role. The prevailing view in epithelial immunology is that \u03b3\u03b4 T lymphocytes regulate tissue homeostasis only after recognizing self-antigens that become exposed upon injury or infection.<\/p>

    However, our previous studies\u2014using a novel combination of intravital confocal microscopy and immunofluorescence techniques (Chodaczek et al., Nature Immunology<\/em> 2012)\u2014demonstrated that skin-resident \u03b3\u03b4 T cells remain in a continuously activated state due to constitutive phosphorylation of the \u03b3\u03b4 TCR, which physically immobilizes them within the epidermis.<\/p>

    Contrary to the widely accepted hypothesis, this suggests the continuous expression of specific antigens in healthy tissue. It remains unknown whether similar mechanisms apply to other epithelia also populated by \u03b3\u03b4 T cells. In the case of the reproductive mucosa, its immune regulation is poorly characterized despite many years of intensive research on immune responses to HIV infection.<\/p>

    The proposed project aims to fill this knowledge gap by:<\/p>

    1. determining the activation status of mucosal T lymphocytes,<\/p><\/li>

    2. assessing their impact on epithelial function and the induction of immune responses, and<\/p><\/li>

    3. visualizing the dynamics of cellular processes in real time, including cell motility and intercellular interactions.<\/p><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

      Project funded by the National Science Centre (NCN) under the \u201cSONATA BIS 4\u201d call Total project value: PLN 1,997,904.00Total funding: PLN 1,340,184.00Project implementation period: 01\/07\/2015 \u2013 30\/06\/2020Principal Investigator: Dr. Grzegorz Chodaczek Project consortium: Wroc\u0142aw Research Centre EIT+ Ltd. \u2013 Consortium Leader Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences The aim […]<\/p>\n","protected":false},"featured_media":94100,"menu_order":0,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","inline_featured_image":false,"footnotes":""},"categories":[727],"grupy_badawcze":[614],"pozostale_projekty":[],"class_list":["post-92989","projects","type-projects","status-publish","format-standard","has-post-thumbnail","hentry","category-finished","grupy_badawcze-grupa-badawcza-immunoterapii"],"acf":{"kierownik_projektu":["Dr. hab. Grzegorz Chodaczek"],"opis_projektu":"","dofinansowanie":"Narodowe Centrum Nauki","nazwa_projektu":"","tematyka_projektu":"","grupa_badawcza":["Immunotherapy Research Group"],"dofinanowanie":"National Science Centre"},"publishpress_future_action":{"enabled":false,"date":"2026-05-01 20:42:19","action":"category","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/92989","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects"}],"about":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/projects"}],"version-history":[{"count":5,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/92989\/revisions"}],"predecessor-version":[{"id":96759,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/92989\/revisions\/96759"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media\/94100"}],"wp:attachment":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=92989"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/categories?post=92989"},{"taxonomy":"grupy_badawcze","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/grupy_badawcze?post=92989"},{"taxonomy":"pozostale_projekty","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pozostale_projekty?post=92989"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}