{"id":98016,"date":"2025-07-28T13:50:29","date_gmt":"2025-07-28T11:50:29","guid":{"rendered":"https:\/\/port.lukasiewicz.gov.pl\/?post_type=projects&#038;p=98016"},"modified":"2026-01-16T09:25:12","modified_gmt":"2026-01-16T08:25:12","slug":"creation-of-a-library-of-plasmid-vectors-inducing-selected-point-mutations-in-the-tp53-gene-sequence-using-prime-editing-technology-wektpred","status":"publish","type":"projects","link":"https:\/\/port.lukasiewicz.gov.pl\/en\/projekty-badawcze\/creation-of-a-library-of-plasmid-vectors-inducing-selected-point-mutations-in-the-tp53-gene-sequence-using-prime-editing-technology-wektpred\/","title":{"rendered":"Creation of a library of plasmid vectors inducing selected point mutations in the TP53 gene sequence using Prime Editing technology \u2013 WEKTPRED"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"98016\" class=\"elementor elementor-98016 elementor-83127\" data-elementor-post-type=\"projects\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2ab4d67 e-flex e-con-boxed e-con e-parent\" data-id=\"2ab4d67\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-77a7f71 elementor-widget elementor-widget-text-editor\" data-id=\"77a7f71\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"26\" data-end=\"255\">Project funded by the National Science Centre (NCN) under the \u201cMINIATURA 7\u201d call<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-24608dd elementor-widget elementor-widget-image\" data-id=\"24608dd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"580\" height=\"50\" src=\"https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-1024x89.png\" class=\"attachment-large size-large wp-image-92145\" alt=\"NCN logo en\" srcset=\"https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-1024x89.png 1024w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-300x26.png 300w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-768x67.png 768w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-1536x134.png 1536w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-2048x178.png 2048w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-1200x104.png 1200w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-1980x172.png 1980w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-24x2.png 24w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-36x3.png 36w, https:\/\/port.lukasiewicz.gov.pl\/wp-content\/uploads\/sites\/35\/2025\/04\/ncn-logo-poziom-en-48x4.png 48w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4e948f00 cut-off-wrapper-90 projects-short-info e-con-full e-flex e-con e-child\" data-id=\"4e948f00\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-1449c456 e-con-full e-flex e-con e-child\" data-id=\"1449c456\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4ac3937f elementor-widget elementor-widget-text-editor\" data-id=\"4ac3937f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Project number:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-55fd9c24 e-con-full e-flex e-con e-child\" data-id=\"55fd9c24\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3f90b04d elementor-widget elementor-widget-text-editor\" data-id=\"3f90b04d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Project value:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-429e6923 elementor-widget elementor-widget-text-editor\" data-id=\"429e6923\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t49,169.00 PLN\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-5c45bb7e e-con-full e-flex e-con e-child\" data-id=\"5c45bb7e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-227b1468 elementor-widget elementor-widget-text-editor\" data-id=\"227b1468\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Funding amount:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1f56194b e-con-full e-flex e-con e-child\" data-id=\"1f56194b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1e320440 elementor-widget elementor-widget-text-editor\" data-id=\"1e320440\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Project implementation period:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2f7d34d8 elementor-widget elementor-widget-text-editor\" data-id=\"2f7d34d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t08.08.2023 \u2013 07.08.2024\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-25bb5337 e-con-full e-flex e-con e-child\" data-id=\"25bb5337\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7ee9ac47 elementor-widget elementor-widget-text-editor\" data-id=\"7ee9ac47\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Project manager:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-63fb6c44 elementor-widget elementor-widget-text-editor\" data-id=\"63fb6c44\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tDr. Ma\u0142gorzata Szatkiewicz\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5be580f elementor-widget elementor-widget-text-editor\" data-id=\"5be580f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"510\" data-end=\"958\">Mutations within the TP53 gene sequence are present in many cancers, as the product of TP53\u2014the p53 protein\u2014is one of the most important tumor suppressor proteins. To facilitate research focused on selected mutations, it is beneficial to create model cell lines exhibiting specific genetic defects. Currently, one of the most precise and efficient methods for inducing mutations in DNA is the new generation of CRISPR\/Cas9 technology\u2014Prime Editing.<\/p><p data-start=\"960\" data-end=\"1442\" data-is-last-node=\"\" data-is-only-node=\"\">The aim of this research project is to create a library of plasmid vectors capable of introducing modifications into the TP53 gene using the Prime Editing method. The prepared plasmids will be used in the future to generate a collection of cell lines derived from stem cells that harbor selected mutations within the TP53 gene sequence. Such cell lines will enable observation of tumor phenotype development as well as efficient and precise testing of the effects of selected drugs.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Project funded by the National Science Centre (NCN) under the \u201cMINIATURA 7\u201d call Project number: Project value: Funding amount: Project implementation period: Project manager: Mutations within the TP53 gene sequence are present in many cancers, as the product of TP53\u2014the p53 protein\u2014is one of the most important tumor suppressor proteins. To facilitate research focused on [&hellip;]<\/p>\n","protected":false},"featured_media":98000,"menu_order":0,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","inline_featured_image":false,"footnotes":""},"categories":[],"grupy_badawcze":[618],"pozostale_projekty":[],"class_list":["post-98016","projects","type-projects","status-publish","format-standard","has-post-thumbnail","hentry","grupy_badawcze-grupa-badawcza-dynamiki-genomu"],"acf":{"nr_projektu":"2023\/07\/X\/NZ1\/00283","nazwa_projektu":"49,169.00 PLN","wartosc_dofinansowania":"49,169.00 PLN","tematyka_projektu":"08.08.2023 \u2013 07.08.2024","opis_projektu":"","grupa_badawcza":["Biology of Astrocytes Research Group"],"kierownik_projektu":["Dr. Ma\u0142gorzata Szatkiewicz"],"dofinansowanie":"Narodowe Centrum Nauki","dofinanowanie":"National Science Centre"},"publishpress_future_action":{"enabled":false,"date":"2026-04-21 16:36:00","action":"category","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/98016","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects"}],"about":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/projects"}],"version-history":[{"count":5,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/98016\/revisions"}],"predecessor-version":[{"id":98024,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/projects\/98016\/revisions\/98024"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media\/98000"}],"wp:attachment":[{"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=98016"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/categories?post=98016"},{"taxonomy":"grupy_badawcze","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/grupy_badawcze?post=98016"},{"taxonomy":"pozostale_projekty","embeddable":true,"href":"https:\/\/port.lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/pozostale_projekty?post=98016"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}