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In this project, we aim to investigate the molecular basis of HIV-1 antisense transcripts associated with its latency. We will manipulate barcoded wildtype HIV-1 and conduct experiments using several “omics” technologies, including B-HIVE, RNA-seq, ChIP-seq, ATAC-seq, MNase-seq, HIV-1-capture HiC followed by high-throughput sequencing to characterize the molecular microenvironments of the latent HIV reservoir, especially those harboring proviruses at a deeper level of latency. Lastly, we will integrate these multidimensional datasets generated in this project to establish a deep learning-based quantitative model, enabling the prediction of the likelihood of the molecular microenvironments of the latent HIV reservoir. The candidate will be involved in above in daily task.
CODZIENNE ZADANIA:
Viral manipulation, including viral transduction and transfection
Cell culture
Perform molecular biology techniques, including PCR, RT-PCR, qPCR, and prepare high-throughput sequencing libraries
FACS sorting
Participate in writing the manuscript
Participate lab activities (e.g., lab meeting and journal club and so on)
Data analysis, interpretation, visualization
Keeping an industry-standard work documentation.
WYMAGANIA OBOWIĄZKOWE:
Sc. degree (or final year of MSc studies) in biological sciences or related field supported with at least 1 year of hands-on laboratory experience.
Excellent interpersonal and communication skills.
Very good work organization and time management.
Experienced to work in interdisciplinary teams.
Conscientiousness and accuracy.
Professional level of English
Experience in cell culture.
Experience in molecular biology techniques (molecular cloning, PCR, RT-PCR, qPCR).
Enthusiastic about learning (new knowledge and techniques).
MILE WIDZIANE:
Experience in any “omics” approach.
Experience in generating high-throughput sequencing libraries.
BENEFITY:
Full- time employment on the position of Process Engineer
Scientific and organizational Support
Co-financing for private medical care.
Co-financing for a sports card.
Possibility to join group life insurance.
Co-financing under the Social Benefits Fund (for holidays and Christmas)