Analytical techniques such as HPLC-DAD, HPLC-FLD, and LC-MS are advanced methods used for the simultaneous identification and quantitative analysis of multiple chemical compounds in samples. HPLC-DAD (high-performance liquid chromatography with a diode-array detector) enables the acquisition of absorption spectra across a broad wavelength range, providing precise analysis of chemical composition. HPLC-FLD (liquid chromatography with a fluorescence detector) employs fluorescence detection, allowing exceptionally sensitive determination of selected compounds.

LC-MS (liquid chromatography coupled with mass spectrometry) combines high-resolution chromatographic separation with the ability to identify molecular masses, enabling detailed analyses, including the detection and identification of unknown substances. Together, these methods provide comprehensive, precise, and highly sensitive tools for chemical analysis.