Life Sciences and Biotechnology Center
Bioanalytics Laboratory
Equipments
Our infrastructure includes advanced equipment such as mass spectrometers, liquid chromatographs, NMR spectrometers, confocal microscopes, an electron microscope, spectrophotometers, and pipetting stations.
This allows us to ensure the highest quality of services and support our clients’ innovative research.
Mass spectrometers and liquid chromatographs
High-resolution mass spectrometer Orbitrap Exploris 480 (Thermo Scientific)
- ion sources: HESI, nanoESI
- FAIMS ion mobility module
- coupled with a Vanquish Neo (Thermo Scientific) liquid chromatograph
Low-resolution mass spectrometer LCMS-8045 (Shimadzu), QqQ type
- mass analyzer: triple quadrupole
- ion source: ESI
- fragmentation: CID
- coupled with the ultra-high-performance liquid chromatograph Nexera X2 (Shimadzu)
Ultra-high-performance liquid chromatograph Ultimate 3000 (Thermo Scientific)
- diode array detector (DAD)
- fluorescence detector (FLD)
Mass spectrometer LTQ Orbitrap Elite (Thermo Scientific)
- ion sources: HESI, nanoESI, APCI
- FAIMS ion mobility module
- coupled with Easy nanoLC 1000/Ultimate 3000 (Thermo Scientific) liquid chromatograph
NMR spectrometers
NMR spectrometer Advance III HD 500 MHz (Bruker)
- sample changer for 60 positions
- broadband probes for measurements in solutions: BBI and BBO
- accessory for variable-temperature measurements in the range from –150 to 150 °C (BCU II and N₂(l) dewar)
NMR spectrometer Advance III HD 600 MHz (Bruker)
- ion sources: HESI, nanoESI, APCI
- FAIMS ion mobility module
- coupled with Easy nanoLC 1000/Ultimate 3000 (Thermo Scientific) liquid chromatograph
Spektrometr NMR Advance III HD 700 MHz (Bruker)
- 24-position sample changer
- broadband probe for solution measurements: BBO
- 3-channel probes for solution measurements: TXI (P and N)
- 4-channel cryoprobe for solution measurements: QCI
- variable-temperature accessory for measurements from -150 to 150°C (BCU II and N₂(l) dewar); for the QCI probe the temperature range is -40 to 80°C
Microscopes
Dual-beam scanning electron microscope SEM/FIB Zeiss Auriga 60
- CrossBeam system, Cobra ion column
- Field emission electron gun (FEG), operating at voltages 0.1–30 kV
- Detectors: SE2, InLens, BSD, ESB, STEM, EDS (X-Max Oxford Instruments)
- GIS: Pt, W, H₂O, insulator
- Cryo SEM/FIB module by Quorum Technologies
Electron microscopy sample preparation
- ultramicrotome Leica EM UC7 with Leica FC7 cryochamber
- high-vacuum coater Leica EM ACE 600
- critical-point dryer Leica EM CPD 300
confocal microscope Leica SP8 MP
- simple stand
- conventional and resonant scanner (8 kHz)
- VIS lasers: 405, 488, 552, 638 nm
- IR laser: Chameleon Vision II, femtosecond pulses, wavelength tunable in the range 680–1080 nm
- detectors: 4 internal PMTs, 1 HyD detector, 4 external NDD-PMTs
- objectives: 10x (NA 0.40), 20x (NA 0.75), 25x (NA 0.95 W), 40x (NA 1.10 W; NA 1.3 oil), 63x (NA 1.4 oil)
- motorized scanning stage
- temperature controller and heating insert for Petri dishes
- set for long-term imaging of laboratory mice
Zeiss Cell Observer SD confocal microscope
- inverted stand
- Yokogawa CSU-X1A 5000 spinning disk module
- lasers: 405, 488, 561, 639 nm
- 2 kamery EMCCD QImaging Rolera EM-C2 (Dual Camera)
- objectives: 10x (NA 0.30), 20x (NA 0.4 LD), 25x (NA 0.80 W), 40x (NA 0.6 LD; NA 1.20 W; NA 1.4 Oil), 63x (NA 1.20 W; NA 1.4 Oil), 100x (NA 1.46 Oil)
- motorized scanning stage with Definite Focus module
- XLmulti S1 incubator, heating insert for 96-well plates
- temperature and O₂/CO₂ concentration controller