Creation of a library of plasmid vectors inducing selected point mutations in the TP53 gene sequence using Prime Editing technology – WEKTPRED

Mutations within the TP53 gene sequence are present in many cancers, as the product of TP53—the p53 protein—is one of the most important tumor suppressor proteins. To facilitate research focused on selected mutations, it is beneficial to create model cell lines exhibiting specific genetic defects. Currently, one of the most precise and efficient methods for inducing mutations in DNA is the new generation of CRISPR/Cas9 technology—Prime Editing.

The aim of this research project is to create a library of plasmid vectors capable of introducing modifications into the TP53 gene using the Prime Editing method. The prepared plasmids will be used in the future to generate a collection of cell lines derived from stem cells that harbor selected mutations within the TP53 gene sequence. Such cell lines will enable observation of tumor phenotype development as well as efficient and precise testing of the effects of selected drugs.