„The role of extracellular microvesicles released by mesenchymal stem cells isolated from umbilical cord in intercellular regulation of oxidative stress”
Project supported by the National Science Centre, Poland under the „MINIATURA 1” programme
Total cost: 49 995,00 PLN
Centre Contribution:49 995,00 PLN
Duration: 08/11/2017 – 07/11/2018
Principal Investigator: dr Jakub Grzesiak
The role of microvesicles and the mechanisms of their action are still unsufficiently explained. In order to utilize their potential in the treatment of degenerative diseases, it is necessary to evaluate how microvesicles induce changes in physiology in recipient cell. Only revealing these mechanisms may lead to development of methods to obtain desired populations of microvesicles, that may be found effective in treating certain diseases (type II diabetes, kidney failure, lung diseases, degenerative joint diseases, neurodegenerative disorders). The project aims at verification of hypotheses, which states that microvesicles released by umbilical cord mesenchymal stem cells contain the transcripts of NFE2L2 gene – the main switch of anti-oxidative response in cells. Delivering of this transcript by microvesicles to the recipient cell results its translation using recipient cell’s ribosomes into the functional protein. The occurence of NFE2L2 protein in the recipient cell induce the anti-oxidative pathway by engaging the downstream proteins (ie. ARE – antioxidative response elements). The confirmation of this hypothesis may lead to development of novel therapeutic strategy that bases on externally-induced activation of cytoprotective mechanisms in cells exposed to high level of oxidative stess (eg. in pancreatic beta cells, in which in the course of type II diabetes the acummulation of negative effects resulted from oxidative stress may lead to the development of type I diabetes).